Trade Agreement: CETA/WTO-AGP/CPTPP/CFTA/FTAs with Peru/Colombia/Panama/Korea/UK Tendering Procedures: Generally only one firm has been invited to bid Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address:
Sony Biotechnology, Inc. 1730 N. 1st Street San Jose California United States 95112 Nature of Requirements:
Fully Automated Cell Sorter System
31184-221232/A Trotechaud, Amber Telephone No. - (873) 455-3495
Advance Contract Award Notice
1.Advance Contract Award Notice (ACAN)
An ACAN is a public notice indicating to the supplier community that a department or agency intends to award a contract for goods, services or construction to a pre-identified supplier, thereby allowing other suppliers to signal their interest in bidding, by submitting a statement of capabilities. If no supplier submits a statement of capabilities that meets the requirements set out in the ACAN, on or before the closing date stated in the ACAN, the contracting officer may then proceed with the award to the pre-identified supplier.
2. Definition of Requirement
The National Research Council (NRC) of Canada has a requirement to purchase a Fully Automated Cell Sorter System, to support the needs of interdisciplinary research within multiple projects using a highly variable user-base. The system will support research and development within the Human Health Therapeutics (HHT) Research Centre related to academic, clinical, and industrial activities.
The System will be used in a biosafety approved environment, which is to be used by a broad range of non-specialist users with limited operational expertise. This will be accomplished by acquiring a system that incorporates disposable microfluidic flow cell and sorting nozzle chips together with a robust, fully automated and traceable cell sorter platform.
The System must include the following:
1. One Cell Sorter; 2. One Fluidics cart; 3. One A2 Class biosafety cabinet with a built-in enclosure compliant with NSF49 biosafety standards including an Aerosol Management System; 4. All accessories including but not limited to start-up reagents, flow cell/ nozzles, cables and power cords to operate the system;
5. Installation and Training; 6. Related operating software and workstation
3. Criteria for Assessment of the Statement of Capabilities (Minimum Essential Requirements)
Any interested supplier must demonstrate by way of a statement of capabilities that its system meets the following requirements.
1. The System must have three lasers consisting of a violet (405nm @ 120mW or higher), blue (488nm @75mW or higher), and yellow/green (561nm @ 65mW or higher);
2. The System must be field upgradable to add an additional 4th red laser (638nm @100mW or higher);
3. The System must have 12 free form photomultiplier tubes (PMTs) supporting 5 fluorescence detectors from the 488nm laser and 7 fluorescence detectors from the 405nm laser;
4. The System must use operator exchangeable (remove and replace) and disposable microfluidic chip with integrated flow cell and nozzles; at least 3 different nozzle on chip sizes (70, 100, and 130um);
5. The System must have a wizard-based fully automated system to perform daily flow cell-laser alignments and delay calibrations, drop size and stream calibrations;
6. The System must use interchangeable nozzles (70, 100, and 130um) that are automatically recognized and calibrated and can be swapped out 3-4 times per day;
7. The System must use automation for instrument performance quality control and sorting run traceability via chip-based bar codes to track date, chip nozzle size, user, and run settings;
8. The System must actively monitor and adjust drop characteristics and break off point during sorting runs;
9. The System must have software controlled on board sample mixing;
10. The System must have software controlled cell deposition that precisely directs and sorts into the center of receiving tubes or wells;
11. The System must have an integrated temperature regulation system (Peltier) without the need for separate fluidics to maintain desired temperatures throughout the sample sort path (input and sort deposition);
12. The System must accommodate a fourth laser (638nm) to run concurrently with three other lasers violet (405nm), blue (488nm), and yellow/green (561nm);
13. The System must detect up to 12 fluorescent colors + 2 additional scatter parameters based on a flexible combination of 3-4 lasers together with 12 PMT detectors and filters;
14. The System must use 3 different nozzle sizes (70um, 100um, and 130um nozzle apertures) allowing user to optimize flow sorting applications to balance between higher pressure faster sorting runs versus lower pressure sorting runs that enhances viable cell recoveries;
15. The System must clear clogs at the nozzle level through software driven settings;
16. The system must automatically identify the placement of each specific fluidic tank and monitor their respective fluid levels, the flow cells, and the nozzle chips;
17. The System must achieve >98% purity and >80% recovery at a threshold rate of 12,000 events per second using a 70um nozzle at 51kHz;
18. The System must adjust vibration frequencies for the 100um standard nozzle size to modify drop volumes allowing for refinement of sorting speeds in line with user defined acceptable purities;
19. The System must allow the exchange of the sample delivery path (sample inlet lines, flow cell, and nozzle system) in a time-limited fashion (
